半胱氨酸丰富血管生成诱导因子61(CYR61)重组蛋白

半胱氨酸丰富血管生成诱导因子61(CYR61)重组蛋白

Recombinant Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61)

CCN1; GIG1; IGFBP10; CCN family member 1; Insulin-like growth factor-binding protein 10

[ PROPERTIES ]

Residues: Asp176~Asp379

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P18406

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 9.3

Predicted Molecular Mass: 26.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

DDLLG LDASEVELTR NNELIAIGKG SSLKRLPVFG TEPRVLFNPL HAHGQKCIVQTTSWSQCSKS CGTGISTRVT NDNPECRLVK ETRICEVRPC GQPVYSSLKK GKKCSKTKKSPEPVRFTYAG CSSVKKYRPK YCGSCVDGRC CTPLQTRTVK MRFRCEDGEMFSKNVMMIQS CKCNYNCPHP NEASFRLYSL FNDIHKFRD

[ REFERENCES ]

1. O'Brien T.P., et al. (1990) Mol. Cell. Biol. 10:3569-3577.

2. Latinkic B.V., et al. (1991) Nucleic Acids Res. 19:3261-3267.

3. Chen N., et al. (2000) J. Biol. Chem. 275:24953-24961.

4. Chen N., et al. (2004) J. Biol. Chem. 279:44166-44176.

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