组蛋白结合核自身抗原精子蛋白(NASP)重组蛋白

组蛋白结合核自身抗原精子蛋白(NASP)重组蛋白

Recombinant Nuclear Autoantigenic Sperm Protein, Histone Binding (NASP)

[ PROPERTIES ]

Residues: Thr3~Asp223

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q66HD3

Host: E. coli

Subcellular Location: Cytoplasm, nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 4.3

Predicted Molecular Mass: 27.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

TESTATAA IAAELVSADK VEDAPAPSTS ADKMESLDVD SEAKKLLGLG QKHLVMGDIPAAVNAFQEAA SLLGKKYGET ANECGEAFFF YGKSLLELAR MENGVLGNAL EGVHVEEEEGEKTEDESLVE NNDNVDEEAR EELREQVYDA MGEKEAKKAE GQSLTKPETD KEQESEVEKGGREDMDISEP AEKLQEKVES TSKQLTESSE EAKEAAIPGL NED

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