Ⅵ型胶原α1(COL6a1)重组蛋白

Ⅵ型胶原α1(COL6a1)重组蛋白

Recombinant Collagen Type VI Alpha 1 (COL6a1)

COL6-A1; OPLL; Collagen Alpha-1(VI)chain

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Thr816~Arg1002

Tags: N-terminal His-Tag

Tissue Specificity: Brain Lung Heart. Subcellular Location: Secreted, extracellular space, extracellular matrix. Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 21.5kDa

Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions. [ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0

mg/mL. Do not vortex. [ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months.

 Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[ SEQUENCE ]

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