布鲁东氏酪氨酸激酶(Btk)重组蛋白

布鲁东氏酪氨酸激酶(Btk)重组蛋白

Recombinant Bruton'S Tyrosine Kinase (Btk)

AGMX1; AT; ATK; BPK; IMD1; PSCTK1; XLA; Bruton Agammaglobulinemia Tyrosine Kinase; B-cell progenitor kinase

[ PROPERTIES ]

Residues: Met1~Gln168

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: E9PTF7

Host: E. coli

Subcellular Location: Cytoplasmic vesicle.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.4

Predicted Molecular Mass: 49.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MAAVILESIF LKRSQQKKKT SPLNFKKRLF LLTVHKLSYY EYDFERGGSK GWRRYSHAREKNYCVENILP PKIPPTRDQV LQRRGEESSE MEQISIIERF PYPFQVVYDE GPLYVFSPTEELRKRWIHQL KNVIRYNSDL VQKYHPCFWI DGQYLCCSQT AKNAMGCQ

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