肌纤蛋白(MYOC)重组蛋白

肌纤蛋白(MYOC)重组蛋白

Recombinant Myocilin (MYOC)

GLC1A; GPOA; JOAG; JOAG1; TIGR; Trabecular Meshwork Inducible Glucocorticoid Response; Myocilin 55 kDa subunit

[ PROPERTIES ]

Residues: Ala224~Tyr471 (Accession # Q99972),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Rough endoplasmic

reticulum. Secreted. Cell projection, cilium.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 29.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF- ASRILKE SPSGYLRSGE GDTGCGELVW VGEPLTLRTA ETITGKYGVWMRDPKPTYPY TQETTWRIDT VGTDVRQVFE YDLISQFMQG YPSKVHILPR PLESTGAVVYSGSLYFQGAE SRTVIRYELN TETVKAEKEI PGAGYHGQFP YSWGGYTDID LAVDEAGLWVIYSTDEAKGA IVLSKLNPEN LELEQTWETN IRKQSVANAF IICGTLYTVS SYTSADATVNFAYDTGTGIS KTLTIPFKNR Y

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