组织蛋白酶K(CTSK)重组蛋白

组织蛋白酶K(CTSK)重组蛋白

Recombinant Cathepsin K (CTSK)

CTS-K; CTS02; CTSO; CTSO1; CTSO2; PKND; PYCD; Pycnodysostosis; Cathepsin O; Cathepsin X

[ PROPERTIES ]

Residues: Pro116~Met329 (Accession # P61277),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Lysosome.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 8.8

Predicted Molecular Mass: 24.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-PDSVD YRKKGYVTPV KNQGQCGSCW AFSSVGALEG QLKKKTGKLLNLS PQ N LV D C VSE ND G CG G G YM T NAFQYV Q KNR GI DSE DA YPYV GQE ES CMYNPTGKAAK CRGYREIPEG NEKALKRAVA RVGPVSVAID ASLTSFQFYS KGVYYDESCNSDNLNHAVLA VGYGIQKGNK HWIIKNSWGE NWGNKGYILM ARNKNNACGI ANLASFPKM

[ REFERENCES ]

1. Guay J., et al. (1999) Bone 25:205-209. 

2. Yamashita D.S., et al. (2006) J. Med. Chem. 49:1597-1612. 

3. Motyckova G., Fisher DE. (2003) Curr. Mol. Med. 2 (5): 407–21.

4. Troen BR. (2006) Ann. N. Y. Acad. Sci. 1068: 165–72

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