白细胞激活黏附因子(ALCAM)重组蛋白

白细胞激活黏附因子(ALCAM)重组蛋白

Recombinant Activated Leukocyte Cell Adhesion Molecule (ALCAM)

CD166; MEMD; Activated Leucocyte Cell Adhesion Molecule

[ PROPERTIES ]

Residues: Trp28~Lys527

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9BH13

Host: E. coli

Subcellular Location: Membrane; Single-pass

type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 59.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

WYT VNSAYGDTII MPCRVDVPQN LMFGKWKYEK PDGSPVFIAF RSSTKKSVQYDDVPEYKDRL NLSENYTLSI SNAKISDEKR FVCMLVTEDD VFEAPTVVKV FKQPSKPEIVKAPFLETDK LKKLGECISK DSYPDGNITW YRNGKVLQAL EGAVVIIFRK QMDSVTQLYTMTSSLEYKTT KADIQMPFTC SVTYYGPSGQ KTVYSEQAVF DIYYPTEQVT IQVLPSKNAIKEGDNITLKC LGNGNPPPEE FLFYLPGQPE GIRSSNTYTL TDVKRNATGD YKCSLVDKKS

MIASAAITVH YLDLSLNPSG EVTKQIGDAL PVSCTISASR NATVVWMKDN VKLRSSPSFSSLHYQDAGNY VCETALQEVE GLKKRESLTL IVEGKPQIKM TKKTDPSGLS KTIICHVEGFPKPAIQWTIT GSGSVINQTE ESPYINGRYY SKIIISPEEN VTLTCTAENQ LERTVNSLNVSAISIPEHDE ADEISDENKE KVNDQAK

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