NANOG同源框蛋白(NANOG)重组蛋白

NANOG同源框蛋白(NANOG)重组蛋白

Recombinant NANOG Homeobox Protein (NANOG)

Homeobox transcription factor Nanog

[ PROPERTIES ]

Residues: Met1~Val305

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q9H9S0

Host: E. coli

Subcellular Location: Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.3

Predicted Molecular Mass: 64.6kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDLLIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYLSLQQMQELSN ILN LSYKQVK TWFQNQRMKS KRWQK NNWPK NS NGVTQKASAPTYPS LYSS YH QGC LV NPT GN LPMWS NQT WNNSTWS NQT QNIQSWS NHSWNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQP NSPA SD LEAA LEAAGEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDV

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