信号转导衔接蛋白1(STAP1)重组蛋白

信号转导衔接蛋白1(STAP1)重组蛋白

Recombinant Signal Transducing Adaptor Protein 1 (STAP1)

BRDG1; STAP1; BCR Downstream Signaling 1; Docking protein BRDG1; Stem cell adaptor protein 1

[ PROPERTIES ]

Residues: Met1~Ala297

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q5BK11

Host: E. coli

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 7.6

Predicted Molecular Mass: 38.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MMAKKPPKPA PRRIFQERLK ITALPLYFEG FLLVKRSDYQ EYEHYWTELR GTTLFFYTDKKSTVYVGKLD IIDLVCLTGQ NSTEKNCAKF TLVLPKEEVH LKTENTESGE EWRGFILTVTELSVPQHVSL LPGQVIRLHE VLEREKKRRT ETDQLPVMPV EKEKEPIQGY ADALNPLPECFYAVSRKEAT EMLEKNPSLG NMILRPGSDS KNYSITIRQE IEMPRIKHFK VICTGKNYTIELEKPVTLPN LFSVIDYFVK ETRGNLRPFI YSADDNFGEE PNMEERSEKL KKSPHKA

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