骨骼肌慢肌肌钙蛋白T(TNNT1)重组蛋白

骨骼肌慢肌肌钙蛋白T(TNNT1)重组蛋白

Recombinant Troponin T Type 1, Slow Skeletal (TNNT1)

ANM; STNT; TNT; TNTS; Slow Skeletal Muscle Troponin T

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Met1~Gly259

Tags: N-terminal His-Tag

Tissue Specificity: Skeletal Muscle. Purity: >92%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and Proclin300. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive

Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 34.2kDa

Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions. Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as

follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition. 

[ SEQUENCE ]

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