钠尿肽受体3(NPR3)重组蛋白

钠尿肽受体3(NPR3)重组蛋白

Recombinant Natriuretic Peptide Receptor 3 (NPR3)

GUCY2B; NPRC; ANPRC; Guanylate Cyclase C; Atrionatriuretic Peptide Receptor C; Atrial natriuretic peptide clearance receptor

[ PROPERTIES ]

Residues: Ala45~Pro467 (Accession # P41740)

linked with SNHKSSVDKLAAALEHHHHHH, with

two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Membrane; Single-pass

type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 53.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS - AQKIEV LVLLPRDDSYLFSLARVRPA IEYALRSVEG NGTGRKLLPP GTRFQVAYED SDCGNRALFS LVDRVAAARGAKPDLILGPV CEYAAAPVAR LASHWDLPML SAGALAAGFQ HKDTEYSHLT RVAPAYAKMGEMMLALFRHH HWSRAALLYS DDKLERNCYF TLEGVHEVFQ EEGLHTSAYN FDETKDLDLDDIVRYIQGSE RVVIMCASGD TIRRIMLAVH RHGMTSGDYA FFNIELFNSS SYGDGSWKRGDKHDFEAKQA YSSLQTVTLL RTAKPEFEKF SMEVKSSVEK QGLNEEDYVN MFVEGFHDAILLYVLALHEV LRAGYSKKDG GKIIQQTWNR TFEGIAGQVS IDANGDRYGD FSVVAMTDTEA G T Q E V I G D Y F G K E G R F K M R S N V K Y P W G S L K L R I D E T R I V E H T N S S PSNHKSSVDKLAAALEHHHHHH

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