二氢硫辛酸脱氢酶(DLD)重组蛋白

二氢硫辛酸脱氢酶(DLD)重组蛋白

Recombinant Dihydrolipoyl Dehydrogenase (DLD)

LAD; GCSL; Dihydrolipoamide Dehydrogenase; E3 Component Of Pyruvate Dehydrogenase Complex; 2-Oxo-Glutarate Complex; Branched Chain Keto Acid Dehydrogenase Complex

[ PROPERTIES ]

Residues: Thr45~Lys271

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: O08749

Host: E. coli

Subcellular Location: Mitochondrion matrix.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.1

Predicted Molecular Mass: 54.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

TVIGSG PGGYVAAIKS AQLGFKTVCI EKNETLGGTC LNVGCIPSKA LLNNSHYYHMAHGKDFASRG IEIPEVRLNL EKMMEQKHSA VKALTGGIAH LFKQNKVVHV NGFGKITGKNQVTATKADGS TQVIDTKNIL VATGSEVTPF PGITIDEDTI VSSTGALSLK KVPEKLVVIGAGVIGVELGS VWQRLGADVT AVEFLGHVGG IGIDMEISKN FQRILQRQGF K

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