FK506结合蛋白样蛋白(FKBPL)重组蛋白

FK506结合蛋白样蛋白(FKBPL)重组蛋白

Recombinant FK506 Binding Protein Like Protein (FKBPL)

NG7; WISP39; DIR1; WAF-1/CIP1 stabilizing protein 39

[ PROPERTIES ]

Residues: Met1~Ser347

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: O35450

Host: E. coli

Subcellular Location: Endoplasmic reticulum

membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 7.6

Predicted Molecular Mass: 68.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

METSPISPMN EKNTAQPQQR EENAQQILNT AIPFRQRSPG LLPEALKVGV RPDPANQIVETQEIEHPVAG FEGDSDQFQV STNEMAEHLQ ASDLWYCPDG SFVKKIIVPG HGLDKPKLGSKCQVLALGFP FGSGMPEGWT ELTIGIGQWR EKMWGELMEK CLESMRQGEE AKIHLPGSSAPLAKLRLDSF TNGRDSWEME AMEKEALAKE EHRRGTELFR AGNPQGAARC YGRALRLLLTLPPPGPPERT TLYANLAACQ LLLGHPQLAA QSCDRVLERE PGHLKALYRR GVARAALGDL

EKATADFKKV LAVDPKNRAA KEELGKVVIQ GRKQDAGLAR GLRKMFS

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