ⅣD组磷脂酶A2(PLA2G4D)重组蛋白

ⅣD组磷脂酶A2(PLA2G4D)重组蛋白

Recombinant Phospholipase A2, Group IVD (PLA2G4D)

cPLA2delta; Cytosolic phospholipase A2 delta

[ PROPERTIES ]

Residues: Pro9~Leu120

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q86XP0

Host: E. coli

Subcellular Location: Cytoplasm. Cytoplasmic

vesicle membrane; Peripheral membrane protein;

Cytoplasmic side.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 4.7

Predicted Molecular Mass: 42.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

PP GHPYQGEAST CWQLTVRVLE ARNLRWADLL SEADPYVILQ LSTAPGMKFKTKTLTDTSHP VWNEAFRFLI QSQVKNVLEL SIYDEDSVTE DDICFKVLYD ISEVLPGKLL

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