70kDa热休克蛋白1样蛋白(HSPA1L)重组蛋白

70kDa热休克蛋白1样蛋白(HSPA1L)重组蛋白

Recombinant Heat Shock 70kDa Protein 1 Like Protein (HSPA1L)

HSP-A1L; HSPA1-L; HSP70-HOM; Hum70t

[ PROPERTIES ]

Source: Prokaryotic expression.

 Host: E. coli

Residues: Met1~Asp641

Tags: N-terminal His-Tag

Purity: >97%

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 60µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.1

Predicted Molecular Mass: 72.1kDa

Accurate Molecular Mass: 44&70kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved

to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in ddH2O to a concentration of 0-0.5 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. 2 / 4

 Store at 2-8ºC for one month.

 Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined

by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no

obvious degradation and precipitation were observed. The loss rate is less than 5% within the

expiration date under appropriate storage condition

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