顶体蛋白(ACR)重组蛋白

顶体蛋白(ACR)重组蛋白

Recombinant Acrosin (ACR)

ACRS; Preproacrosin

[ PROPERTIES ]

Residues: Ile43~Gly291

Tags: N-terminal His-Tag

Accession: P29293

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.0

Predicted Molecular Mass: 29.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

IVGGQTSS PGAWPWMVSL QIFTSHNSRR YHACGGSLLN SHWVLTAAHC FDNKKKVYDWRLVFGAHEIE YGRNKPVKEP QQERYVQKIV IHEKYNAVTE GNDIALLKVT PPVTCGDFVGPGCLPHFKSG PPRIPHTCYV TGWGYIKDNA PRPSPVLMEA RVDLIDLDLC NSTQWYNGRVTSTNVCAGYP EGKIDTCQGD SGGPLMCRDS VDSPFVIVGI TSWGVGCARA KRPGVYTATWDYLDWIASKI G

[ REFERENCES ]

1. Klemm U., et al. (1991) Biochim. Biophys. Acta 1090:270-272. 

2. Kremling H., et al. (1991) DNA Seq. 2:57-60. 

3. Kwitek A.E., et al. (2004) Genome Res. 14:750-757. 

4. Szpirer C., et al. (1998) Mamm. Genome 9:721-734.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！