C-Ros癌基因1(ROS1)重组蛋白

C-Ros癌基因1(ROS1)重组蛋白

Recombinant C-Ros Oncogene 1, Receptor Tyrosine Kinase (ROS1)

MCF3; ROS; c-Ros-1; v-Ros Avian UR2 Sarcoma Virus Oncogene Homolog 1; Proto-oncogene tyrosine-protein kinase ROS; Receptor tyrosine kinase c-ros oncogene 1

[ PROPERTIES ]

Residues: Leu1937~Phe2210

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q63132

Host: E. coli

Subcellular Location: Cell membrane; Single-pass

type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 34.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

LSLR LLLGSGAFGE VYEGTAVDIL GRGSGEIKVA VKTLKKGSTD QEKIEFLKEAHLMSKFNHPN ILKQLGVCLL SEPQYIILEL MEGGDLLSYL RKARGTTLSG PLLTLADLVELCVDISKGCV YLEQMHFIHR DLAARNCLVS VKDYTSPRVV KIGDFGLARE IYKHDYYRKRGEGLLPVRWM APENLMDGIF TSQSDVWSFG ILVWEILTLG HQPYPAHSNL DVLNYVQAGGRLEPPRNCPD DLWNLMFRCW AQEPDQRPTF YNIQDQLQLF

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