二氢硫辛酸转乙酰基酶(DLAT)重组蛋白

二氢硫辛酸转乙酰基酶(DLAT)重组蛋白

Recombinant Dihydrolipoyl Transacetylase (DLAT)

DLTA; PDC-E2; PBC; M2 antigen complex 70 kDa subunit; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Pyruvate dehydrogenase complex E2

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Thr368~Trp626

Tags: N-terminal His-Tag

Tissue Specificity: Brain, Heart, Kidney, Liver. Subcellular Location: Mitochondrion matrix. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 5% trehalose and 0.01% sarcosyl. Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification;

Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.4

Predicted Molecular Mass: 29.0kDa

Accurate Molecular Mass: 29kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved.The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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