过氧化还原酶3(PRDX3)重组蛋白

过氧化还原酶3(PRDX3)重组蛋白

Recombinant Peroxiredoxin 3 (PRDX3)

MER5; AOP-1; AOP1; SP-22; Antioxidant Protein 1; Thioredoxin-dependent peroxide reductase, mitochondrial

[ PROPERTIES ]

Residues: Val5~Trp233

Tags: N-terminal His-Tag

Accession: P30048

Host: E. coli

Subcellular Location: Mitochondrion.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 10mM

PBS, pH7.4, containing 1mM DTT, 5% trehalose,

0.01% sarcosyl and preservative.

Predicted isoelectric point: 6.9

Predicted Molecular Mass: 26.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VGRLLR ASVARHVSAI PWGISATAAL RPAACGRTSL TNLLCSGSSQ AKLFSTSSSCHAPAVTQHAP YFKGTAVVNG EFKDLSLDDF KGKYLVLFFY PLDFTFVCPT EIVAFSDKANEFHDVNCEVV AVSVDSHFSH LAWINTPRKN GGLGHMNIAL LSDLTKQISR DYGVLLEGSGLALRGLFIID PNGVIKHLSV NDLPVGRSVE ETLRLVKAFQ YVETHGEVCP ANW

[ REFERENCES ]

1. Cao Z., et al. (2007) J. Mol. Biol. 372:1022-1033.

2. Masaki M., et al. (2003) Eur. J. Biochem. 270:76-83.

3. Wagner E., et al. (2002) Biochem. J. 366:777-785.

4. Tsuji K., et al. (1995) Biochem. J. 307:377-381.

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