卵巢肿瘤蛋白1(OTUB1)重组蛋白

卵巢肿瘤蛋白1(OTUB1)重组蛋白

Recombinant Otubain 1 (OTUB1)

HSPC263; OTB1; OTU1; OTU Domain,Ubiquitin Aldehyde Binding 1; Deubiquitinating enzyme OTUB1; Ubiquitin-specific-processing protease OTUB1

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Otubain 1

Gene Names: OTUB1

Size: 100µg

Source: Recombinant

Expression Expression Expression Expression Host: E.coli

Function: Function: Function: Function: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and

may therefore play an important regulatory role at the level of protein turnover by

preventing degradation. Regulator of T-cell anergy. Acts via its interaction with

RNF128/GRAIL. Surprisingly, it regulates RNF128-mediated ubiquitination. Mediates

deubiquitination of 'Lys-48'-linked polyubiquitin chains. Also capable of removing

NEDD8 from NEDD8 conjugates, but with a nuch lower preference compared to

'Lys-48'-linked ubiquitin. Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Met1~Lys271 (Accession # B2RYG6), with a N-terminal His-tag.

Grade & Purity: Purity: Purity: Purity: >97%, 32.51 kDa as determined by SDS-PAGE reducing conditions.

Form & Buffer: Supplied as lyophilized form in PBS, pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg(determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA;IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 32.51 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Store at 4oC for short term storage (1-2 weeks). Aliquot and store at -20oC or -80oC for long term

storage. Avoid repeated freeze/thaw cycles.

Valid period: period: period: period: 12 months stored at -80oC.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

MGHHHHHHSGS-MAAEEPQQQK QEPLGSDSEG VNCLAYDEAI MAQQDRIQQE IAVQNPLVSE RLELSVLYKEYAEDDNIYQQ KIKDLHKKYS YIRKTRPDGN CFYRAFGFSH LEALLDDSKE LQRFKAVSAK SKEDLVSQGFTEFTIEDFHN TFMDLIEQVE KQTSVADLLA SFNDQSTSDY LVVYLRLLTS GYLQRESKFF EHFIEGGRTV

KEFCQQEVEP MCKESDHIHI IALAQALSVS IQVEYMDRGE GGTTNPHIFP EGSEPKVYLL YRPGHYDILY K

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Maurya D.K., et al. (2009) Proteomics. 9:2593-2599.

2. Strausberg RL, et al. (2002) Proc Natl Acad Sci U S A.99(26):16899-16903.

3. The MGC Project Team. (2004) Genome Res. 14:2121-2127

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