前列腺素F2受体负调节因子(PTGFRN)重组蛋白

前列腺素F2受体负调节因子(PTGFRN)重组蛋白

Recombinant Prostaglandin F2 Receptor Negative Regulator (PTGFRN)

CD315; CD9P-1; EWI-F; FPRP; SMAP-6; CD9 partner 1; Glu-Trp-Ile EWI motif-containing protein F; Prostaglandin F2-alpha receptor-associated protein

[ PROPERTIES ]

Residues: Pro406~Pro662

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9P2B2

Host: E. coli

Subcellular Location: Endoplasmic reticulum

membrane, Single-pass type I membrane protein.

Golgi apparatus, trans-Golgi network membrane.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 7.1

Predicted Molecular Mass: 33.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

PDYQV YLNASKVPGF ADDPTELACR VVDTKSGEAN VRFTVSWYYR MNRRSDNVVTSELLAVMDGD WTLKYGERSK QRAQDGDFIF SKEHTDTFNF RIQRTTEEDR GNYYCVVSAWTKQRNNSWVK SKDVFSKPVN IFWALEDSVL VVKARQPKPF FAAGNTFEMT CKVSSKNIKSPRYSVLIMAE KPVGDLSSPN ETKYIISLDQ DSVVKLENWT DASRVDGVVL EKVQEDEFRYRMYQTQVSDA GLYRCMVTAW SP

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