50kDa核孔蛋白(NUP50)重组蛋白

50kDa核孔蛋白(NUP50)重组蛋白

Recombinant Nucleoporin 50kDa (NUP50)

NPAP60; NPAP60L; Nuclear pore-associated protein 60 kDa-like

[ PROPERTIES ]

Residues: Pro179~Val428

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: O08587

Host: E. coli

Subcellular Location: Nucleus, nuclear pore

complex. Nucleus membrane; Peripheral membrane

protein; Nucleoplasmic side.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.5

Predicted Molecular Mass: 56.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

PL CDLTPIFKDY ERYLATIEKQ LENGGSSSSE RQTDRATAAM EPPSLFGSTK LQQDSPFSFHGNKAEDTSEK LEFTAEKKSD AAQGATSASF NFGKKIESSV LGSLSSGSLT GFSFSPGNSSLFGKDAAQSK AASSPFSAKA SESQAGGSSS ECRDGEEEES DEPPKVVVTE VKEEDAFYSKKCKLFYKKDN EFKEKGVGTL HLKPTATQKT QLLVRADTNL GNILLNVLIP PNMPCTRTGKNNVLIVCV

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