脑源性神经营养因子(BDNF)重组蛋白

脑源性神经营养因子(BDNF)重组蛋白

Recombinant Brain Derived Neurotrophic Factor (BDNF)

Neurotrophin; Abrineurin

[ PROPERTIES ]

Residues: Ser147~Leu249

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: O70183

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.2

Predicted Molecular Mass: 44.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

SVCD SVSEWVTAAD KKTAVDMSGG TVTVLEKVPV SKGQLKQYFY ETKCNPMGYTKEGCRGIDKR HWNSQCRTTQ SYVRALTMDS KKRIGWRFIR IDTSCVCTL

[ REFERENCES ]

1. Inoue M., et al. (1998) Submitted to the EMBL/GenBank/DDBJ databases.

2. Robinson RC., et al. (1995) Biochemistry 34 (13): 4139–46.

3. Binder DK., Scharfman HE. (2004) Growth Factors 22 (3): 123–31.

4. Huang EJ., Reichardt LF. (2001) Annual Review of Neuroscience 24: 677–736.

5. Szuhany KL., et al. (2015) Journal of Psychiatric Research 60: 56–64.

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