解整合素金属蛋白酶12(ADAM12)重组蛋白
Recombinant A Disintegrin And Metalloprotease 12 (ADAM12)
MCMP; MCMPMltna; MLTN; MLTNA; Meltrin Alpha; Disintegrin and metalloproteinase domain-containing protein 12
[ PROPERTIES ]
Residues: Ser513~His767
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O43184
Host: E. coli
Subcellular Location: Cell membrane; Single-pass
type I membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.9
Predicted Molecular Mass: 31.3kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
SCQDVDGY CYNGICQTHE QQCVTLWGPG AKPAPGICFE RVNSAGDPYG NCGKVSKSSFAKCEMRDAKC GKIQCQGGAS RPVIGTNAVS IETNIPLQQG GRILCRGTHV YLGDDMPDPGLV LA G T K CA D GKI C L N R Q C Q NI S V F GV HE C AM Q C H G R GV C NN RK N C H CE AHWAPPFCDKF GFGGSTDSGP IRQADNQGLT IGILVTILCL LAAGFVVYLK RKTLIRLLFTNKKTTIEKLR CVRPSRPPRG FQPCQAH
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