T-细胞可诱导共刺激分子(ICOS)重组蛋白
Recombinant Inducible T-Cell Co Stimulator (ICOS)
CD278; AILIM; Inducible T-Cell Costimulator; Activation-Inducible Lymphocyte Immunomediatory Molecule
[ PROPERTIES ]
Source: Prokaryotic expression. Host: E. coli
Residues: Asn35~Ile131
Tags: N-terminal His-Tag
Tissue Specificity: Spleen, Lung, Liver, Brain. Subcellular Location: Membrane; Single-pass type I membrane protein. Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 7.8
Predicted Molecular Mass: 12.2kDa
Accurate Molecular Mass: 12kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8
oC for one month. Aliquot and store at -80
oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]
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