Rac-GTP酶激活蛋白1(RACGAP1)重组蛋白

Rac-GTP酶激活蛋白1(RACGAP1)重组蛋白

Recombinant Rac-GTPase Activating Protein 1 (RACGAP1)

HsCYK-4; ID-GAP; MgcRacGAP; Male germ cell RacGap

[ PROPERTIES ]

Residues: Met106~Trp539

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q9H0H5

Host: E. coli

Subcellular Location: Nucleus, Cytoplasm,

cytoskeleton, spindle, Cytoplasmic vesicle,

secretory vesicle, acrosome, Cleavage furrow,

Midbody, Cell membrane, Peripheral membrane

protein, Cytoplasmic side.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 78.1kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MLMCD TSGSIQLSEE QKSALAFLNR GQPSSSNAGN KRLSTIDESG SILSDISFDKTDESLDWDSS LVKTFKLKKR EKRRSTSRQF VDGPPGPVKK TRSIGSAVDQ GNESIVAKTTVTVPNDGGPI EAVSTIETVP YWTRSRRKTG TLQPWNSDST LNSRQLEPRT ETDSVGTPQSNGGMRLHDFV SKTVIKPESC VPCGKRIKFG KLSLKCRDCR VVSHPECRDR CPLPCIPTLIGTPVKIGEGM LADFVSQTSP MIPSIVVHCV NEIEQRGLTE TGLYRISGCD RTVKELKEKFLRVKTVPLLS KVDDIHAICS LLKDFLRNLK EPLLTFRLNR AFMEAAEITD EDNSIAAMYQAVGELPQANR DTLAFLMIHL QRVAQSPHTK MDVANLAKVF GPTIVAHAVP NPDPVTMLQD

IKRQPKVVER LLSLPLEYW

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