嘌呤能受体P2Y14(P2RY14)重组蛋白

嘌呤能受体P2Y14(P2RY14)重组蛋白

Recombinant Purinergic Receptor P2Y, G Protein Coupled 14 (P2RY14)

GPR105; P2Y14; P2Y Purinoceptor 14; G Protein-Coupled Receptor 105; UDP-glucose receptor

[ PROPERTIES ]

Residues: Pro51~Arg303 (Accession # Q15391),

with two N-terminal Tags, His-tag and T7-tag. Host: E. coli

Subcellular Location: Cell membrane;

Multi-pass membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 9.9

Predicted Molecular Mass: 33.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- PSSKSFIIYL KNIVIADFVMSLTFPFKILG DSGLGPWQLN VFVCRVSAVL FYVNMYVSIV FFGLISFDRY YKIVKPLWTSFIQSVSYSKL LSVIVWMLML LLAVPNIILT NQSVREVTQI KCIELKSELG RKWHKASNYIFVAIFWIVFL LLIVFYTAIT KKIFKSHLKS SRNSTSVKKK SSRNIFSIVF VFFVCFVPYHIARIPYTKSQ TEAHYSCQSK EILRYMKEFT LLLSAANVCL DPIIYFFLCQ PFR

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