谷胱甘肽S转移酶(GST)重组蛋白

谷胱甘肽S转移酶(GST)重组蛋白

Recombinant Glutathione S Transferase (GST)

GST-Tag

[ PROPERTIES ]

Source: Prokaryotic expression 

Host: E.coli 

Residues: Met1~Lys218 

Tags: No tags Subcellular 

Location: Secreted 

Purity: > 95% 

Traits: Freeze-dried powder 

Buffer formulation: PBS, pH7.4, containing 5% Trehalose. 

Original Concentration: 200µg/mL 

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.) 

Predicted isoelectric point: 5.9 

Predicted Molecular Mass: 25.8kDa 

Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions. 

[ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. 

[ SEQUENCE ]