间隙连接蛋白31(CX31)重组蛋白

间隙连接蛋白31(CX31)重组蛋白

Recombinant Connexin 31 (CX31)

GJB3; GJ-B3; DFNA2; EKV; Gap Junction Protein Beta 3; Erythrokeratodermia Variabilis

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Connexin 31

Synonyms Synonyms Synonyms Synonyms: CX31, Gjb3, Cxn-31

Species: Species: Species: Species: Rat

Size: 100µg

Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cell membrane; Multi-pass membrane protein. Cell junction, gap

junction.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Ile210~Ile270 (Accession # P25305), with two N-terminal Tags, His-tag and

GST-tag. Grade & Purity: Purity: Purity: Purity: >95%, 34 kDa as determined by SDS-PAGE reducing conditions.

Formulation Formulation Formulation Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method). Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 33.6 kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 7.7

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obviousdegradation and precipitation were observed. (Referring from China Biological Products Standard, which was

calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date underappropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.

MRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDGSTSGSGHHHHHHSAGLVPRGSTAIGMKETAAAKFERQHMDSPDLGTLEVLFQGPLGSEF-I FHRIMRGLSK DKSTKSISSP

KSSSRASTCR CHHKLLESGD LEAVPADDKL QASAPSLTPI

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