CD40配体(CD40L)重组蛋白

CD40配体(CD40L)重组蛋白

Recombinant Cluster Of Differentiation 40 Ligand (CD40L)

CD154; TRAP; HIGM1; IGM; IMD3; TBAM; T-BAM; TNFSF5; Gp39; TNF Superfamily Member 5; Hyper-IgM Syndrome; TNF-Related Activation Protein; T-Cell B-Cell Activating Molecule

[ PROPERTIES ]

Residues: Lys105~Leu260 (Accession # Q9Z2V2),

with two N-terminal Tags, His-tag and GST-tag. Host: E. coli

Subcellular Location: Cell membrane;

Single-pass type II membrane protein, Secreted. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and preservative.

Predicted isoelectric point: 6.8

Predicted Molecular Mass: 49.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target

protein. The loss rate was determined by accelerated thermal degradation test,

that is, incubate the protein at 37oC for 48h, and no obvious degradation and

precipitation were observed. (Referring from China Biological Products Standard,

which was calculated by the Arrhenius equation.) The loss of this protein is less

than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- KKEKSF EMQRGDEDPQIAAHVVSEAN SNAASVLQWA KKGYYTMKSN LVVLENGRQL TVKREGLYYV YTQVTFCSNREPLSQRPFIV SLWLKPSSGS ERILLRAANT HSSSKLCEQQ SIHLGGVFEL QAGASVFVNV

TEASQVIHGI GFSSIGLLKL

[ REFERENCES ]

1. Li Y., et al. (2010) Mol Oral Microbiol 25:275-292.

2. Chew M., et al. (2010) Inflamm. Res. 59:979-982.

3. Nesbeth Y.C., et al. (2010) J. Immunol. 184:5654-5662.

4. Iida T., et al. (2010) Surgery 148:925-935.

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