cGMP依赖性蛋白激酶Ⅱ(PRKG2)重组蛋白

cGMP依赖性蛋白激酶Ⅱ(PRKG2)重组蛋白

Recombinant Protein Kinase, cGMP Dependent Type II (PRKG2)

PRKGR2; cGKII; cGMP-dependent protein kinase 2

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Leu453~Leu728

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Intestine, Prostate Gland. Subcellular Location: Apical cell membrane; Lipid-anchor. Purity: >94%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 61.8kDa

Accurate Molecular Mass: 62kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date underappropriate storage condition.

[ SEQUENCE ]

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