基质金属蛋白酶1(MMP1)重组蛋白

基质金属蛋白酶1(MMP1)重组蛋白

Recombinant Matrix Metalloproteinase 1 (MMP1)

CLGN; CLG1; Collagenase; Interstitial Collagenase; Vertebrate Collagenase; Fibroblast Collagenase

[ PROPERTIES ]

Residues: Asn169~Cys464

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: B5DFD5

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 4.8

Predicted Molecular Mass: 37.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

NP FDGPEYGLAH TFPPGPRLGG DVHYDLDETW TDNSDNFNLF YVTAHELGHSLGLTHSRDVG ALMFPSYTWY TEDFVLNQED INRIQALYGP SPNPIQPTGA TTPHPCDGDLTFDAITTFRG EVMFFKGRFY IRVNKFMPEA ELNLIGIIWP NLPGKLDAAY EVSMIDQVRYFKGNKVWVVR EHTVLRGFPT DIHSFFGFPS SVTHIDAAVC EEETGKTYFF VDNMYWRYDENTQSMDPGYP RLTAEDFPGI DDKVDDVFQK GENFYFFHQS VQRRFSLRIR RVDDSRDSSTWFNC

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