细胞色素P450家族成员2E1(CYP2E1)重组蛋白

细胞色素P450家族成员2E1(CYP2E1)重组蛋白

Recombinant Cytochrome P450 2E1 (CYP2E1)

CPE1; CYPIIE1; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; Cytochrome P450 Family 2 Subfamily E 1; Cytochrome P450,Subfamily IIE(Ethanol-Inducible)Polypeptide 1

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Cytochrome P450 2E1

Synonyms: Synonyms: Synonyms: Synonyms: CYP2E1, Cyp2e, Cyp2e-1

Species: Species: Species: Species: Rat

Size: 100µg

Source: Source: Source: Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Endoplasmic reticulum

membrane; Peripheral membrane protein. Microsome

membrane.

[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]

Residues: Residues: Residues: Residues: Arg126~Arg309 (Accession # P05182),

with N-terminal His-Tag. Grade & Purity: Purity: Purity: Purity: >95%, 25kDa as determined by

SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH

7.4, containing 5% sucrose.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by

the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 23.1kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 7.1

Rat CYP2E1 kDa

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-RRFSL SILRDWGMGK QGNEARIQRE AQFLVEELKK TKGQPFDPTFLIGCAPCNVI ADILFNKRFD YNDKKCLRLM SLFNENFYLL STPWIQLYNN FADYLRYLPGSHRKIMKNVS EIKQYTLEKA KEHLQSLDIN CARDVTDCLL IEMEKEKHSQ EPMYTMENVSVTLADLFFAG TETTSTTLR

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