HAUS Augmin样复合体亚基7(HAUS7)重组蛋白

HAUS Augmin样复合体亚基7(HAUS7)重组蛋白

Recombinant HAUS Augmin Like Complex Subunit 7 (HAUS7)

UCHL5IP; UIP1; UCHL5 Interacting Protein; UCH37 Interacting Protein 1; 26S Proteasome-Associated UCH Interacting Protein 1; X-linked protein STS1769

[ PROPERTIES ]

Residues: Met1~Leu227 (Accession # Q8BKT8),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

Spindle.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 4.9

Predicted Molecular Mass: 26.8kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSG SEF-MAEEGAGAGS DDSYYKDVGD DCVVKAAVEV FEKLKGVNCPFLDGLYITEP KTIMELLCRP SKYRLDILEW MCIRVCPSLQ DKFISLKGNA LDLKIQEMLKLGHELMFCAP DDQDLLMGRE CPQKQLQFMD KILDMMQSLA TGCSSSSSLK EHLEDTTEKNEALLGELFSS PNLRAILKPE SDPWPLDMQS SLNKQSDDLP KADPSAQSEG EKVADLARQLQESATKL

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