糖原磷酸化酶L(PYGL)重组蛋白

糖原磷酸化酶L(PYGL)重组蛋白

Recombinant Glycogen Phosphorylase, Liver (PYGL)

GPLL; GPBB; Hers Disease; Glycogen Storage Disease Type VI

[ PROPERTIES ]

Residues: Thr341~Gly509

Tags: N-terminal His-Tag

Accession: P09811

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 1mM DTT, 5% trehalose, 0.01%

sarcosyl and preservative.

Predicted isoelectric point: 7.8

Predicted Molecular Mass: 21.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

THPALAIPEL MRIFVDIEKL PWSKAWEITK KTFAYTNHTV LPEALERWPV DLVEKLLPRHLQIIYEINQK HLDRIVALFP KDIDRMRRMS LIEEEGGKRI NMAHLCIVGC HAVNGVAKIHSDIVKTQVFK DFSELEPDKF QNKTNGITPR RWLLLCNPGL ADLIAEKIG

[ REFERENCES ]

1. Armstrong C.G., et al. (1998) Biochem. J. 336:699-704.

2. Doherty M.J., et al. (1995) FEBS Lett. 375:294-298.

3. Moorhead G., et al. (1995) FEBS Lett. 362:101-105.

4. Froman B.E., et al. (1991) Gene 109:269-274.

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