骨粘连蛋白(ON)重组蛋白

骨粘连蛋白(ON)重组蛋白

Recombinant Osteonectin (ON)

SPARC; BM-40; Secreted Protein,Acidic,Cysteine-Rich; Basement-membrane protein 40; Secreted protein acidic and rich in cysteine

DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Osteonectin

Synonyms: Synonyms: Synonyms: Synonyms: ON

Size: 100 µg

Source: Source: Source: Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted, extracellular space, extracellular matrix, basement

membrane.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Ala18~Ile302 (Accession # P07214), with a N-terminal his-tag.

Grade & Purity: Purity: Purity: Purity: >95%, 33.8 kDa as determined by SDS-PAGE reducing conditions.

Formulation: Formulation: Formulation: Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 33.8 kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 4.9

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in PBS, PH7.2-PH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months .

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was

determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious

degradation and precipitation were observed. (Referring from China Biological Products Standard, which was

calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under

appropriate storage condition.

[ BACKGROUND BACKGROUND BACKGROUND BACKGROUND]

The target protein is fused with a N-terminal his-tag. Their sequence is listed below.

MGHHHHHHSGS-APQ QTEVAEEIVE EETVVEETGV PVGANPVQVE MGEFEDGAEE TVEEVVADNP CQNHHCKHGKVCELDESNTP MCVCQDPTSC PAPIGEFEKV CSNDNKTFDS SCHFFATKCT LEGTKKGHKL HLDYIGPCKY IAPCLDSELTEFPLRMRDWLKNVLVTLYER DEGNNLLTEK QKLRVKKIHE NEKRLEAGDH PVELLARDFE KNYNMYIFPV HWQFGQLDQHPIDGYLSHTE LAPLRAPLIP MEHCTTRFFE TCDLDNDKYI ALEEWAGCFG IKEQDINKDL VI

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Villarreal XC., et al. (1989) Biochemistry 28 (15): 6483–91.

2. Guweidhi A., et al. (2005) Ann. Surg. 242 (2): 224–234.

3. Wasi S., et al. (1984) Can. J. Biochem. Cell Biol. 62 (6): 470–8.

4. Young MF., et al. (1992) Clin. Orthop. Relat. Res. (281): 275–94.

5. Lane TF., et al. (1994) FASEB J. 8 (2): 163–73

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