氢离子转运ATP酶线粒体F1复合体β肽(ATP5b)重组蛋白

氢离子转运ATP酶线粒体F1复合体β肽(ATP5b)重组蛋白

Recombinant ATPase, H+ Transporting, Mitochondrial F1 Complex Beta Polypeptide (ATP5b)

ATPMB; ATPSB

[ PROPERTIES ]

Residues: Ala47~Ser529

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P10719

Host: E. coli

Subcellular Location: Mitochondrion.

Mitochondrion inner membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.0

Predicted Molecular Mass: 55.4kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

AAQS SAAPKAGTAT GQIVAVIGAV VDVQFDEGLP PILNALEVQG RESRLVLEVAQHLGESTVRT IAMDGTEGLV RGQKVLDSGA PIKIPVGPET LGRIMNVIGE PIDERGPIKTKQFAPIHAEA PEFIEMSVEQ EILVTGIKVV DLLAPYAKGG KIGLFGGAGV GKTVLIMELINNVAKAHGGY SVFAGVGERT REGNDLYHEM IESGVINLKD ATSKVALVYG QMNEPPGARARVALTGLTVA EYFRDQEGQD VLLFIDNIFR FTQAGSEVSA LLGRIPSAVG YQPTLATDMGTMQERITTTK KGSITSVQAI YVPADDLTDP APATTFAHLD ATTVLSRAIA ELGIYPAVDPLDSTSRIMDP NIVGSEHYDV ARGVQKILQD YKSLQDIIAI LGMDELSEED KLTVSRARKIQRFLSQPFQV AEVFTGHMGK LVPLKETIKG FQQILAGDYD HLPEQAFYMV GPIEEAVAKADKLAEEHGS

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