酸性成纤维细胞生长因子(FGF1)重组蛋白

酸性成纤维细胞生长因子(FGF1)重组蛋白

Recombinant Fibroblast Growth Factor 1, Acidic (FGF1)

ECGF; AFGF; ECGFA; ECGFB; FGF-Alpha; FGFA; HBGF1; ECGFB; HBGF1; Acidic Fibroblast Growth Factor; heparin-binding growth factor 1; Endothelial Cell Growth Factor, Beta

[ PROPERTIES ]

Source: Prokaryotic expression.

Host: E. coli

Residues: Phe16~Asp155

Tags: N-terminal His-Tag

Subcellular Location: Secreted. Cytoplasm, cell cortex. Cytosol. Nucleus. Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA,

1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 7.2

Predicted Molecular Mass: 17.1kDa

Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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