免疫缺陷伴血小板减少综合征蛋白(WASP)重组蛋白

免疫缺陷伴血小板减少综合征蛋白(WASP)重组蛋白

Recombinant Wiskott Aldrich Syndrome Protein (WASP)

WAS; IMD2; THC; Eczema-Thrombocytopenia

[ PROPERTIES ]

Residues: Met1~Gly298

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: M0RBE8

Host: E. coli

Subcellular Location: Cell-cell junction, extracellular

vesicular exosome, vesicle membran.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 6.7

Predicted Molecular Mass: 36.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MSSGPVGGRS GGRGGPAVQQ NIPSNLLQDH ENQRLFELLG RKCWTLATAV VQLYLALPPGAEHWTMEHCG AVCFVKDNPQ KSYFIRLYGL QAGRLLWEQE LYSQLVYLTP TPFFHTFAGDDCQVGLNFSD ESEAQAFRAL VQEKIQKRNQ RQSGERRQLP LTPAPTNEER RGGLPPVPPHPGGDHGGPSG GPLSLGLVTV DIQNPDITSS RYRGLPAPGP GPTDKKRSGK KKISKADIGAPSGFKHVSHV GWDPQNGFDV NNLDPDLRSL FSRAGISEAQ LTDAETSKLI YDFIEDQG

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