血管紧张素转化酶(ACE)重组蛋白

血管紧张素转化酶(ACE)重组蛋白

Recombinant Angiotensin I Converting Enzyme (ACE)

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]

Protein Protein Protein Protein Names: Angiotensin I Converting Enzyme

Synonyms Synonyms Synonyms Synonyms: ACE, CD143

Species: Species: Species: Species: Rabbit

Size: 100 µg

Source: Escherichia coli-derived

Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Angiotensin-converting enzyme, soluble form: Secreted.

Cell membrane; Single-pass type I membrane protein.

[ PROPERTIES ROPERTIES ROPERTIES ROPERTIES ]

Residues Residues Residues Residues: Tyr498~Asn715 (Accession # P12822), with N-terminal His-Tag.

Grade & Purity: Purity: Purity: Purity: >97%, 26.6 kDa as determined by SDS-PAGE reducing conditions.

Formulation Formulation Formulation Formulation: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.

Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by the LAL method).

Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 26.6 kDa

Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 5.4

[ PREPARATION PREPARATION PREPARATION PREPARATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]

Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months .

Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obviousdegradation and precipitation were observed. (Referring from China Biological Products Standard, which wascalculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date underappropriate storage condition.

[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES]

The target protein is fused with N-terminal His-tag, its sequence is listed below.

YLR TKYQGICPPV VRNETHFDAG AKFHIPSVTP YIRYFVSFVL QFQFHQALCM EAGHQGPLHQ CDIYQSTRAGAKLRAVLQAG CSRPWQEVLK DMVASDALDA QPLLDYFQPV TQWLQEQNER NGEVLGWPEY QWRPPLPNNYPEGIDLVTDE AEASRFVEEY DRSFQAVWNE YAEANWNYNT NITTEASKIL LQKNMQIANH TLTYGNWARR FDVSN

[ REFERENCES REFERENCES REFERENCES REFERENCES ]

1. Turner AJ., et al. (2002) Can. J. Physiol. Pharmacol. 80 (4): 346–53.

2. Turner AJ., et al. (2004) Trends Pharmacol. Sci. 25 (6): 291–4.

3. Katovich MJ., et al. (2005) Exp. Physiol. 90 (3): 299–305.

4. Ferrario CM., et al. (2006) Am. J. Physiol. Heart Circ. Physiol. 289 (6): H2281–90.

5. Jia HP., et al. (2006) Adv. Exp. Med. Biol.. Advances in Experimental Medicine and Biology 581: 479–84.

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