肌球蛋白重链2(MYH2)重组蛋白

肌球蛋白重链2(MYH2)重组蛋白

Recombinant Myosin Heavy Chain 2 (MYH2)

MYH2A; MYHSA2; MYHas8; MyHC-2A; MyHC-IIa; Myosin Heavy Chain 2, Skeletal Muscle, Adult

[ PROPERTIES ]

Source: Prokaryotic expression. Host: E. coli

Residues: Leu1237~Ala1471

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Skeletal Muscle. Subcellular Location: Cytoplasm, myofibril. Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 57.1kDa

Accurate Molecular Mass: 57kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. 

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8

oC for one month. Aliquot and store at -80

oC for 12 months. 

Stability Test: The thermal stability is described by the loss rate. The loss ratewas determined by accelerated thermal degradation test, that is, incubate theprotein at 37oC for 48h, and no obvious degradation and precipitation wereobserved. The loss rate is less than 5% within the expiration date under

appropriate storage condition.

[ SEQUENCE ]

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