含KH域RNA结合信号通路关联蛋白1(KHDRBS1)重组蛋白

含KH域RNA结合信号通路关联蛋白1(KHDRBS1)重组蛋白

Recombinant KH Domain Containing, RNA Binding, Signal Transduction Associated Protein 1 (KHDRBS1)

Sam68; p62; GAP-Associated Tyrosine Phosphoprotein p62; Src-associated in mitosis 68 kDa protein; p21 Ras GTPase-activating protein-associated p62

[ PROPERTIES ]

Residues: Lys152~Glu381

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q07666

Host: E. coli

Subcellular Location: Nucleus. Membrane.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.5

Predicted Molecular Mass: 28.9kDa

Accurate Molecular Mass: 36kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

KNMKLKERV LIPVKQYPKF NFVGKILGPQ GNTIKRLQEE TGAKISVLGK GSMRDKAKEEELRKGGDPKY AHLNMDLHVF IEVFGPPCEA YALMAHAMEE VKKFLVPDMM DDICQEQFLELSYLNGVPEP SRGRGVPVRG RGAAPPPPPV PRGRGVGPPR GALVRGTPVR GAITRGATVTRGVPPPPTVR GAPAPRARTA GIQRIPLPPP PAPETYEEYG YDDTYAEQSY E

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