内皮素转化酶2(ECE2)重组蛋白

内皮素转化酶2(ECE2)重组蛋白

Recombinant Endothelin Converting Enzyme 2 (ECE2)

Methyltransferase-like region

[ PROPERTIES ]

Residues: Met1~Phe163

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q80Z60

Host: E. coli

Subcellular Location: Golgi Apparatus Membrane.

Single-pass Type Ii Membrane Protein. Cytoplasmic

Granule Membrane..

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 4.3

Predicted Molecular Mass: 21.9kDa

Accurate Molecular Mass: 15&22kDa as determined by SDS-PAGE reducing

conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to

give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MASP RTP VSP PE LP EK NFQY RQVQYW DQ RY KDAA DS GPYE WFG DFASF RALLEPELCPED RILVLGCGNS ALSYELFLGG FPNVTSVDYS PVVVAAMQVR YAHVPSLRWETMDVRALDFP SGSFDVVLEK GTLDAMLAGE PDPWNVSSEG VHTVDQVLSE VGF

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