Vav3癌基因(VAV3)重组蛋白

Vav3癌基因(VAV3)重组蛋白

Recombinant Vav 3 Oncogene (VAV3)

Vav 3 Guanine Nucleotide Exchange Factor

[ PROPERTIES ]

Residues: His183~Glu380

Tags: N-terminal His-Tag

Accession: Q9UKW4

Host: E. coli

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 10mM

PBS, pH7.4, containing 1mM DTT, 5% trehalose,

0.01% sarcosyl and preservative.

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 24.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

HQPKCPEN DIRSCCLAEI KQTEEKYTET LESIEKYFMA PLKRFLTAAE FDSVFINIPELVKLHRNLMQ EIHDSIVNKN DQNLYQVFIN YKERLVIYGQ YCSGVESAIS SLDYISKTKEDVKLKLEECS KRANNGKFTL RDLLVVPMQR VLKYHLLLQE LVKHTTDPTE KANLKLALDAMKDLAQYVNE VKRDNETLRE

[ REFERENCES ]

1. Movilla N., Bustelo X.R. (1999) Mol. Cell. Biol. 19:7870-7885.

2. Zeng L., et al. (2000) Mol. Cell. Biol. 20:9212-9224.

3. Yabana N., Shibuya M. (2002) Oncogene 21:7720-7729.

4. Hunter S.G., et al. (2006) Mol. Cell. Biol. 26:4830-4842.

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