钙激活核苷酸酶1(CANT1)重组蛋白

钙激活核苷酸酶1(CANT1)重组蛋白

Recombinant Calcium Activated Nucleotidase 1 (CANT1)

SCAN1; SHAPY; CAN1; Soluble Ca-Activated Nucleotidase,Isozyme 1; Apyrase homolog; Putative MAPK-activating protein PM09; Putative NF-kappa-B-activating protein 107

[ PROPERTIES ]

Residues: Val111~Ile403

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q8VCF1

Host: E. coli

Subcellular Location: Endoplasmic reticulum. Golgi

apparatus. Membrane. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 5.1

Predicted Molecular Mass: 36.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

VIADLDTGSR AQEENTWFSY LKKGYLTLSD SGDRVSVEWD KDHGVLESHL AEKGRGMELSDLIVFNGKLY SVDDRTGVIY QIEGTKAVPW VILSDGDGTV EKGFKAEWLA VKDEHLYVGGLGKEW T T T TG EVM NE NPEWV KV VG HRGSV D HE NWV SSY NA LRAAA GI RPPGYLIHESACW SDTLQRWFFL PRRASHERYS EKDDERKGSN LLLSAAQDFR DISVRQVGTLIPTHGFSSFK FIPNTDDQII VALKSEEDNG RIATYVMAFT LDGRFLLPET KIGTVKYEGI EFI

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