半胱氨酸丰富分泌蛋白1(CRISP1)重组蛋白

半胱氨酸丰富分泌蛋白1(CRISP1)重组蛋白

Recombinant Cysteine Rich Secretory Protein 1 (CRISP1)

AEGL1; ARP; CRISP-1; HSCRISP1D; HSCRISP1G; HUMARP; Acidic Epididymal Glycoprotein-like 1; Acidic epididymal glycoprotein homolog; AEG-like protein

[ PROPERTIES ]

Residues: Gln20~His246

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P12020

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 4.9

Predicted Molecular Mass: 29.5kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

Q DT T DEW D RD L EN LST TK LS V QEEII NK HN Q LR RTVSPS GS DL L RVEW DH DAYVNAQKWAN RCIYNHSPLQ HRTTTLKCGE NLFMANYPAS WSSVIQDWYD ESLDFVFGFGPK KV GV KV G H YTQV VW NST F LVA C GVAE CP DQP LK YF YV C HY CP G G NYV GRLY S P YT E GE PC DS CP G N CE DG L CT NS CEY ED NY S N CG D L KKMV S C D DP LLKEGCRASCF CEDKIH

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