肝细胞癌相关蛋白1(HCRP1)重组蛋白

肝细胞癌相关蛋白1(HCRP1)重组蛋白

Recombinant Hepatocellular Carcinoma Related Protein 1 (HCRP1)

PQBP2; VPS37A; Vacuolar Protein Sorting 37 Homolog A; Polyglutamine Binding Protein 2; ESCRT-I complex subunit VPS37A

[ PROPERTIES ]

Residues: Met1~Leu397

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q8NEZ2

Host: E. coli

Subcellular Location: Late endosome membrane;

Peripheral membrane protein. Nucleus.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.4

Predicted Molecular Mass: 48.0kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MSWLFPLTKS ASSSAAGSPG GLTSLQQQKQ RLIESLRNSH SSIAEIQKDV EYRLPFTINNLTININILLP PQFPQEKPVI SVYPPIRHHL MDKQGVYVTS PLVNNFTMHS DLGKIIQSLLDEFWKNPPVL APTSTAFPYL YSNPSGMSPY ASQGFPFLPP YPPQEANRSI TSLSVADTVSSSTTSHTTAK PAAPSFGVLS NLPLPIPTVD ASIPTSQNGF GYKMPDVPDA FPELSELSVSQLTDMNEQEE VLLEQFLTLP QLKQIITDKD DLVKSIEELA RKNLLLEPSL EAKRQTVLDKYELLTQMKST FEKKMQRQHE LSESCSASAL QARLKVAAHE AEEESDNIAE DFLEGKMEIDDFLSSFMEKR TICHCRRAKE EKLQQAIAMH SQFHAPL

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