氯离子通道辅助蛋白1(CLCA1)重组蛋白

氯离子通道辅助蛋白1(CLCA1)重组蛋白

Recombinant Chloride Channel Accessory 1 (CLCA1)

CaCC1; CLCRG1; Chloride Channel,Calcium Activated,Family Member 1; Calcium-activated chloride channel protein 1

[ PROPERTIES ]

Residues: Asn416~Arg670

Tags: N-terminal His-Tag

Accession: A8K7I4

Host: E. coli

Subcellular Location: Secreted, extracellular space.

Cell membrane; Peripheral membrane protein;

Extracellular side.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,

150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,

0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 7.1

Predicted Molecular Mass: 28.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

 Store at 2-8

oC for one month.

 Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

NTISG CFNEVKQSGA IIHTVALGPS AAQELEELSK MTGGLQTYAS DQVQNNGLIDAFGALSSGNG AVSQRSIQLE SKGLTLQNSQ WMNGTVIVDS TVGKDTLFLI TWTMQPPQILLWDPSGQKQG GFVVDKNTKM AYLQIPGIAK VGTWKYSLQA SSQTLTLTVT SRASNATLPPITVTSKTNKD TSKFPSPLVV YANIRQGASP ILRASVTALI ESVNGKTVTL ELLDNGAGAD

ATKDDGVYSR YFTTYDTNGR

[ REFERENCES ]

1. Bailey S.D., et al. (2010) Diabetes Care 33:2250-2253.

2. Hauber H.P., et al. (2010) J. Cyst. Fibros. 9:277-279.

3. Wu W., et al. (2010) Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 24:193-5,2010:199-199.

4. Han S., et al. (2010) Hum. Immunol. 71:727-730.

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