外皮蛋白(EVPL)重组蛋白

外皮蛋白(EVPL)重组蛋白

Recombinant Envoplakin (EVPL)

EVPK; p210; IVL; 210 kDa cornified envelope precursor protein; 210 kDa paraneoplastic pemphigus antigen

[ PROPERTIES ]

Residues: Lys3~Met258

Tags: Two N-terminal Tags, His-tag and GST-tag

Accession: Q92817

Host: E. coli

Subcellular Location: Cell junction. Cytoplasm. Cytoskeleton.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.9

Predicted Molecular Mass: 58.9kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

KGLSKGSQ GKGSPKGSPA KGSPKGSPSR HSRAATQELA LLISRMQANA DQVERDILETQKRLQQDRLN SEQSQALQHQ QETGRSLKEA EVLLKDLFLD VDKARRLKHP QAEEIEKDIKQL HE RVTQE C AEY RA LYE KM VLPP DVGP RV DWA RV LE QKQ KQV CAG QYGPGMAELEQQIA EHNILQKEID AYGQQLRSLV GPDAATIRSQ YRDLLKAASW RGQSLGSLYTHLQGCTRQLS ALAEQQRRIL QQDWSDLM

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