趋化因子C-X3-C-基元配体1(CX3CL1)重组蛋白

趋化因子C-X3-C-基元配体1(CX3CL1)重组蛋白

Recombinant Chemokine C-X3-C-Motif Ligand 1 (CX3CL1)

NTN; ABCD3; C3Xkine; CXC3; CXC3C; NTT; SCYD1; ABCD3; FKN; Neurotactin; Fractalkine; Small Inducible Cytokine Subfamily D(Cys-X3-Cys)Member 1

[ PROPERTIES ]

Residues: Gln83~Pro330 (Accession # O35188),

with N-terminal His-Tag.

Host: E. coli

Subcellular Location: Cell membrane;

Single-pass type I membrane protein.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 4.8

Predicted Molecular Mass: 27.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSG SEF -QDAMKH LD HQAAALTKNG GKFEKRVDNV TPGITLATRGLSPSALTKPE SATLEDLALE LTTISQEARG TMGTSQEPPA AVTGSSLSTS EAQDAGLTAKPQSIGSFEAA DISTTVWPSP AVYQSGSSSW AEEKATESPS TTAPSPQVST TSPSTPEENVGSEGQPPWVQ GQDLSPEKSL GSEEINPVHT DNFQERGPGN TVHPSVAPIS SEETPSPELVASGSQAPKIE EPIHATADPQ KLSVLITPVP

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